Use of multidimentional flow cytometry plots to improve measurable residual disease monitoring of acute myeloid leukaemias
نویسندگان
چکیده
Background: Acute myeloid leukaemia (AML) is a haematological malignancy with prevalence of ∼1.5%.1 Improved AML management requires increasingly sensitive measurable residual disease (MRD) detection, by either molecular or flow cytometric methods.2 We hypothesised that the effect phenotypic changes, well-known feature AML, on multiparameter cytometry MRD (MFC-MRD) analysis can be minimised using multidimensional radar plots. Methods: created novel template plots (Kaluza software). The method was validated 80 consecutive MFC-MRD samples, followed testing cohort (n=36). Results: two methods showed moderate correlation (k=45%) no statistically significant difference (p=0.21). Nineteen tests were positive plot and negative our current method. 14/19 (74%) had low-level MRD, between 0.01% 0.1%. Molecular results available for 36/116 cases only showing slight (k=15%; p=1.0). Discussion: Moderate agreement MFC suggest fit purpose. Discrepancies partially explained increased sensitivity tests. Other possible confounding factors include leukaemic subclones treatment-related changes.3 are currently conducting prospective study to better understand these findings. 1.World Health Organization. 2014 Review Cancer Medicines WHO List Essential Medicines. Cited 1 Apr 2021. http://www.who.int/selection_medicines/committees/ expert/20/applications/AML_APL.pdf.2.Zeijlemaker W, Gratama JW, Schuurhuis GJ. Tumor heterogeneity makes "moving target" detection disease. Cytometry B Clin Cytom 2014; 86: 3–14.3.Li Morgan R, Nieder et al. Normal reactive minor cell populations in bone marrow peripheral blood mimic minimal leukemia cytometry. 2020; Nov 16: 10.1002/cyto.b.21968.
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ژورنال
عنوان ژورنال: Pathology
سال: 2021
ISSN: ['1465-3931', '0031-3025']
DOI: https://doi.org/10.1016/j.pathol.2021.06.096